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gfp reporter genes pfab4282  (Biofab Inc)

 
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    Biofab Inc gfp reporter genes pfab4282
    Unigems plasmids
    Gfp Reporter Genes Pfab4282, supplied by Biofab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+reporter+genes+pfab4282/gfp+reporter+genes+pfab4282/pmc10569648-79-2-29
    Average 90 stars, based on 1 article reviews
    gfp reporter genes pfab4282 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Unigems: plasmids and parts to facilitate teaching on assembly, gene expression control and logic in E. coli"

    Article Title: Unigems: plasmids and parts to facilitate teaching on assembly, gene expression control and logic in E. coli

    Journal: Access Microbiology

    doi: 10.1099/acmi.0.000596.v3

    Unigems plasmids
    Figure Legend Snippet: Unigems plasmids

    Techniques Used:

    Primers for the unigems plasmids
    Figure Legend Snippet: Primers for the unigems plasmids

    Techniques Used: Sequencing

    GFP fluorescence of three replicates of each p006-strongGFP and p006-weakGFP and RFP yellow fluorescence of p005-strongRFP and p005-weakRFP. Note the difference in scale in GFP vs RFP: the lack of differences between strong and weak RFP fluorescence is due to a mismatch between its optimal excitation wavelength (550 nm) and the 488 nm excitation laser in the flow cytometer (see Discussion).
    Figure Legend Snippet: GFP fluorescence of three replicates of each p006-strongGFP and p006-weakGFP and RFP yellow fluorescence of p005-strongRFP and p005-weakRFP. Note the difference in scale in GFP vs RFP: the lack of differences between strong and weak RFP fluorescence is due to a mismatch between its optimal excitation wavelength (550 nm) and the 488 nm excitation laser in the flow cytometer (see Discussion).

    Techniques Used: Fluorescence, Flow Cytometry

    Images of p005-kan-RFP (left column) and p006-kan-GFP (right column) in daylight ( a ) and on a benchtop UV transilluminator (excitation wavelength 395 nm) ( b ). Transformed DH5α cells were grown with 50 µg ml −1 of kanamycin and induced with 100 mM IPTG. The single RFP-expressing colony on the top right plate is due to a mix of plasmids used by the students in this transformation. Photographs were taken with a mobile phone by JB.
    Figure Legend Snippet: Images of p005-kan-RFP (left column) and p006-kan-GFP (right column) in daylight ( a ) and on a benchtop UV transilluminator (excitation wavelength 395 nm) ( b ). Transformed DH5α cells were grown with 50 µg ml −1 of kanamycin and induced with 100 mM IPTG. The single RFP-expressing colony on the top right plate is due to a mix of plasmids used by the students in this transformation. Photographs were taken with a mobile phone by JB.

    Techniques Used: Transformation Assay, Expressing

    Related Articles

    Control:

    Article Title: Unigems: plasmids and parts to facilitate teaching on assembly, gene expression control and logic in E. coli
    Article Snippet: GFP and RFP reporter genes, placed under the control of either a strong or a weak promoter (pFAB4026 and pFAB4282 for GFP and pFAB4005 and pFAB4024 for RFP, respectively) (BIOFAB collection [ ]) were analysed using a flow cytometer on three replicate samples each (separate colonies from the same transformation event).

    Flow Cytometry:

    Article Title: Unigems: plasmids and parts to facilitate teaching on assembly, gene expression control and logic in E. coli
    Article Snippet: GFP and RFP reporter genes, placed under the control of either a strong or a weak promoter (pFAB4026 and pFAB4282 for GFP and pFAB4005 and pFAB4024 for RFP, respectively) (BIOFAB collection [ ]) were analysed using a flow cytometer on three replicate samples each (separate colonies from the same transformation event).

    Transformation Assay:

    Article Title: Unigems: plasmids and parts to facilitate teaching on assembly, gene expression control and logic in E. coli
    Article Snippet: GFP and RFP reporter genes, placed under the control of either a strong or a weak promoter (pFAB4026 and pFAB4282 for GFP and pFAB4005 and pFAB4024 for RFP, respectively) (BIOFAB collection [ ]) were analysed using a flow cytometer on three replicate samples each (separate colonies from the same transformation event).

    Sequencing:

    Article Title: Unigems: plasmids and parts to facilitate teaching on assembly, gene expression control and logic in E. coli
    Article Snippet: GFP and RFP reporter genes, placed under the control of either a strong or a weak promoter (pFAB4026 and pFAB4282 for GFP and pFAB4005 and pFAB4024 for RFP, respectively) (BIOFAB collection [ ]) were analysed using a flow cytometer on three replicate samples each (separate colonies from the same transformation event).

    Fluorescence:

    Article Title: Unigems: plasmids and parts to facilitate teaching on assembly, gene expression control and logic in E. coli
    Article Snippet: GFP and RFP reporter genes, placed under the control of either a strong or a weak promoter (pFAB4026 and pFAB4282 for GFP and pFAB4005 and pFAB4024 for RFP, respectively) (BIOFAB collection [ ]) were analysed using a flow cytometer on three replicate samples each (separate colonies from the same transformation event).

    Expressing:

    Article Title: Unigems: plasmids and parts to facilitate teaching on assembly, gene expression control and logic in E. coli
    Article Snippet: GFP and RFP reporter genes, placed under the control of either a strong or a weak promoter (pFAB4026 and pFAB4282 for GFP and pFAB4005 and pFAB4024 for RFP, respectively) (BIOFAB collection [ ]) were analysed using a flow cytometer on three replicate samples each (separate colonies from the same transformation event).



    Similar Products

    gfp reporter genes pfab4282  (Biofab Inc)
    90
    Biofab Inc gfp reporter genes pfab4282
    Unigems plasmids
    Gfp Reporter Genes Pfab4282, supplied by Biofab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+reporter+genes+pfab4282/gfp+reporter+genes+pfab4282/pmc10569648-79-2-29
    Average 90 stars, based on 1 article reviews
    gfp reporter genes pfab4282 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Unigems plasmids

    Journal: Access Microbiology

    Article Title: Unigems: plasmids and parts to facilitate teaching on assembly, gene expression control and logic in E. coli

    doi: 10.1099/acmi.0.000596.v3

    Figure Lengend Snippet: Unigems plasmids

    Article Snippet: GFP and RFP reporter genes, placed under the control of either a strong or a weak promoter (pFAB4026 and pFAB4282 for GFP and pFAB4005 and pFAB4024 for RFP, respectively) (BIOFAB collection [ ]) were analysed using a flow cytometer on three replicate samples each (separate colonies from the same transformation event).

    Techniques:

    Primers for the unigems plasmids

    Journal: Access Microbiology

    Article Title: Unigems: plasmids and parts to facilitate teaching on assembly, gene expression control and logic in E. coli

    doi: 10.1099/acmi.0.000596.v3

    Figure Lengend Snippet: Primers for the unigems plasmids

    Article Snippet: GFP and RFP reporter genes, placed under the control of either a strong or a weak promoter (pFAB4026 and pFAB4282 for GFP and pFAB4005 and pFAB4024 for RFP, respectively) (BIOFAB collection [ ]) were analysed using a flow cytometer on three replicate samples each (separate colonies from the same transformation event).

    Techniques: Sequencing

    GFP fluorescence of three replicates of each p006-strongGFP and p006-weakGFP and RFP yellow fluorescence of p005-strongRFP and p005-weakRFP. Note the difference in scale in GFP vs RFP: the lack of differences between strong and weak RFP fluorescence is due to a mismatch between its optimal excitation wavelength (550 nm) and the 488 nm excitation laser in the flow cytometer (see Discussion).

    Journal: Access Microbiology

    Article Title: Unigems: plasmids and parts to facilitate teaching on assembly, gene expression control and logic in E. coli

    doi: 10.1099/acmi.0.000596.v3

    Figure Lengend Snippet: GFP fluorescence of three replicates of each p006-strongGFP and p006-weakGFP and RFP yellow fluorescence of p005-strongRFP and p005-weakRFP. Note the difference in scale in GFP vs RFP: the lack of differences between strong and weak RFP fluorescence is due to a mismatch between its optimal excitation wavelength (550 nm) and the 488 nm excitation laser in the flow cytometer (see Discussion).

    Article Snippet: GFP and RFP reporter genes, placed under the control of either a strong or a weak promoter (pFAB4026 and pFAB4282 for GFP and pFAB4005 and pFAB4024 for RFP, respectively) (BIOFAB collection [ ]) were analysed using a flow cytometer on three replicate samples each (separate colonies from the same transformation event).

    Techniques: Fluorescence, Flow Cytometry

    Images of p005-kan-RFP (left column) and p006-kan-GFP (right column) in daylight ( a ) and on a benchtop UV transilluminator (excitation wavelength 395 nm) ( b ). Transformed DH5α cells were grown with 50 µg ml −1 of kanamycin and induced with 100 mM IPTG. The single RFP-expressing colony on the top right plate is due to a mix of plasmids used by the students in this transformation. Photographs were taken with a mobile phone by JB.

    Journal: Access Microbiology

    Article Title: Unigems: plasmids and parts to facilitate teaching on assembly, gene expression control and logic in E. coli

    doi: 10.1099/acmi.0.000596.v3

    Figure Lengend Snippet: Images of p005-kan-RFP (left column) and p006-kan-GFP (right column) in daylight ( a ) and on a benchtop UV transilluminator (excitation wavelength 395 nm) ( b ). Transformed DH5α cells were grown with 50 µg ml −1 of kanamycin and induced with 100 mM IPTG. The single RFP-expressing colony on the top right plate is due to a mix of plasmids used by the students in this transformation. Photographs were taken with a mobile phone by JB.

    Article Snippet: GFP and RFP reporter genes, placed under the control of either a strong or a weak promoter (pFAB4026 and pFAB4282 for GFP and pFAB4005 and pFAB4024 for RFP, respectively) (BIOFAB collection [ ]) were analysed using a flow cytometer on three replicate samples each (separate colonies from the same transformation event).

    Techniques: Transformation Assay, Expressing